PCR Direto do Tecido

Mais uma inovação na área de biologia molecular... agora pode-se realizar PCR sem prévia extração e/ou purificação de DNA.

Terra™ PCR Direct - Genotype Directly from Tissue!

 

 

Direct PCR allows for genome amplification directly from small tissue samples (muscle, hair, nail, leaf, etc.) or crude extracts, without DNA purification. It saves time, reduces cost, and prevents sample loss.

Terra PCR Direct mixes and kits are optimized for direct PCR and feature a unique non-Taq DNA polymerase and optimized buffers that are designed to tolerate PCR inhibitors. Templates up to 2 kb and 70% GC-content can be amplified with high yields.

 

Two protocols to choose from:

• One-Step: Add your plant/animal tissue sample directly into the PCR vial. This is the fastest protocol!
• Two-Step: Add your tissue sample into an extraction buffer first, then transfer an aliquot into the PCR vial. Remaining aliquots may be used in other experiments, or stored.

   

Terra PCR Direct has been used successfully with the following organisms.

 

Corn	Cow	Human	Spinach	Goat	Mouse
Tomato	Sheep	Zebrafish	Sugar cane	Chicken	Xenopus
Rice	Pig	Drosophila	Cotton	Leishmania	C. elegans
Chestnut	Mycelia	Dragonfly	Arabidopsis	Yeast	Prawn
Moss spores	Soil Bacillus	Trichogramma

 

 FONTE: http://info.clontech.com/JSCSLT_TerraOverviewPageSM.html